Enzymatic activity measurement has a broad application in drug screening, diagnosis and disease staging and molecular profiling. However, conventional immunopeptidemetric assays (IMPA) exhibit low fluorescence signal-to-noise ratios, preventing reliable measurements at lower concentrations in the clinically important pM~nM range.
Berkeley Lab researchers demonstrated a highly sensitive measurement of protease activity using nanoplasmonic resonator (NPR). NPRs enhance Raman signals by 6.1x1010 times in a highly reproducible manner, enabling fast detection of protease and enzyme activity, such as Prostate Specific Antigen (paPSA), in real time, at a sensitivity level of 6 pM (0.2 ng/ml) with a dynamic range of three orders of magnitude.
Experiments on extracellular fluid from paPSA-positive cells demonstrate specific detection in a complex bio-fluid background. This method offers a fast, sensitive, accurate and one-step approach to detect the protease activities in very small sample volumes.
STATUS: Published patent application (WIPO 2009/094058). Available for licensing or collaborative research.
FOR MORE INFORMATION:
Sun, C., Su K.-H., Valentine, J., Rosa-Bauza, Y. T., Ellman, J. A., Elboudware, O., Mukherjee, B., Craik, C. S., Shuman, M. A., Chen, F., Zhang, X., "Time Resolved Single Step Protease Activity Quantification Using Nanoplasmonic Resonator Sensors," ACS Nano, Vol 4., No. 2, 2010.
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REFERENCE NUMBER: JIB-2421