APPLICATIONS OF TECHNOLOGY:
Ulli Weier of Berkeley Lab and colleagues have developed a novel and flexible FISH protocol for use with fluorescence microscopy or Spectral Imaging to significantly reduce the overall cost per pregnancy and increase pregnancy rates following in vitro fertilization. While current FISH protocols characterize a subset of chromosomes, the Berkeley Lab method enables full karyotype analysis of a cell in interphase.
The Berkeley Lab protocols are the first to use up to eight chromosome specific DNA probes simultaneously to complete a full karyotype analysis in repeated rounds of hybridization in time for implantation. This test can identify all numeric chromosome abnormalities, which will reduce the chances of spontaneous abortion from nonmosaic embryos. When performed on polar bodies, the test should also increase the chances of fertilization. At present, the use of eight probes simultaneously requires Spectral Imaging while the use of six probes simultaneously is compatible with conventional fluorescence microscopy or Spectral Imaging. The scoring system can be tailored to individual needs.
While conventional chromosome characterization methods can be applied only to blastomeres and first polar bodies, the Berkeley Lab test can be applied to nuclei from second polar bodies, spermatocytes, and somatic and tumor cells, as well.
FOR MORE INFORMATION SEE:
Weier, H.-U.G., Weier, J.F., Oter R.M., Zheng, X., Colls, P., Nureddin,A., Pham, C.D., Chu, L.W., Racowsky, C., Munné, S., “Fluorescence in situ hybridization (FISH) and spectral imaging (SIm) analysis of human oocytes and first polar bodies,” J. Histochem. Cytochem., 2005, 53:269-272.
REFERENCE NUMBER: IB-1992