SEQUENCING
COST: $20 per sample. If our first attempt to obtain a sequence is
unsuccessful, a second reaction and run will be performed at no additional
charge. If both attempts are unsuccessful, the charge will still be
$20/sample.
HOW TO SUBMIT SAMPLES: Please use the request
form and send your request
by email to Ying Zou at yzou@lbl.gov).
Please do not submit request forms by fax or mail.
Bring your clearly marked samples, together with a hard copy of the
request form, to Building 84, Room 355. You need not call ahead - there is a
clearly marked shelf in a designated freezer in 84-355, and you can simply leave
your samples there.
WHAT TO SUBMIT: The
request form describes the
DNA and primer concentrations and sample volumes that we require (sufficient for
two reactions). The facility will provide T3, T7, Universal and Reverse primers.
All other primers must be provided by the requesting investigator.
Please provide the samples and primers in the exact concentrations and
volumes specified on the request form. We strongly recommend that
your DNA samples be purified by Qiagen or Promega kits or similar means.
If you use a simple mini-prep procedure, please provide a picture of a gel
on which the sample is run along side a standard. This will help us
trouble-shoot if the first sequencing reaction fails.
QUESTIONS: Please call (x2217) or email
(yzou@lbl.gov) Ying Zou if you have
any questions.
We are pleased to announce that the LSD Cell Genotyping
and Phenotyping (CG/P) core facility is now accepting RNA
samples for microarray hybridization and analysis by
special software on a charge-back basis.
WHAT THE FACILITY WILL DO:
OPTION TWO: The facility will take hybridized membranes and generate the phosphor images using our Cyclone phosphorimager or just the phosphor images and analyze them with our software. Simply supply a pair of matched phosphorimages or scanned autoradiographs in TIFF format. The data will be stored on a CD for you and sent via email as well.
COST: $1,800 per HYBRIDIZATION experiment (analysis of two
samples), which start with RNA samples to report and CD deliver to
you. $199.00 per comparison of two images that you create in TIFF
format.
HOW TO SUBMIT SAMPLES: Please use the microarray service request form and send
your request by email to Ying Zou at YZOU@LBL.GOV. Bring your
*clearly marked* samples on dry ice together with a hard copy of
the request form, to Building 84, Room 355. You need not call ahead
- there is a clearly marked shelf in a designated -80C freezer in
84-355, and you can simply leave your samples there.
WHAT TO SUBMIT: The enclosed form describes the RNA
concentrations and sample volumes that we require (sufficient for
two reactions) include a photograph (total RNA) of your samples,
along with OD readings.
QUESTIONS: Please email Ying Zou at YZOU@LBL.GOV or call (X2217)
if you have any questions.
MORE CG/P INFO: The CG/P facility was established by an LDRD grant last
year. Our long term aim is to provide sequencing (including SAGE) and microarray
analyses to LSD investigators. Ying is the manager of the CG/P facility, and she is
working with Goberdhan Dimri, the facility's molecular biologist, to develop
and optimize the microarray and SAGE analyses. The facility is co-directed
by Judy Campisi, Paul Kaufman and Kunxin Luo.
OPTION ONE: The facility will take total RNA samples or poly A + RNA samples, hybridize them to Clontech Atlas human or mouse cDNA expression arrays (each with 1,176 or 588 genes), generate phosphor images of the hybridized membranes, and analyze them using AtlasImage software. The data will be presented as a report in the form of an Excel Spreadsheet. This report will show the relative signal itensities and include the comparisons of the signal intensities of two samples. It will be stored on a CD for you and sent via email as well.