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EHSS
Waste Management

EHS
Waste Management

9. Biohazardous Waste Disposal at JGI/PGF

The JGI does not produce any regulated medical waste from the research that is conducted there, thus the biohazardous waste that is produced there is managed differently from the medical/biohazardous waste produced in Berkeley. JGI has autoclaves that are used to sterilize waste contaminated with biohazardous material. Examples of the biohazardous wastes produced at JGI include attenuated E. coli, bacteria, and recombinant DNA. The autoclaves at JGI employ saturated steam under a pressure of approximately 15 psi to achieve a chamber temperature of at least 121C (250F) for at least 40 minutes.

 

9.1 JGI Autoclave Procedures

        Waste contaminated only with biohazardous material is deposited into designated waste containers in the lab marked with the following sign, Biological Contaminated Waste ONLY to be AUTOCLAVED (i.e., E. coli, bacteria, recombinant DNA). Waste contaminated with hazardous chemicals does not belong in this type of container. Contact your Safety Coordinator or Waste Management Generator Assistant for guidance on disposal of waste with chemical contaminants.

        Each biologically contaminated waste container is lined with 2 clear autoclave bags.

        When the container is full, remove and seal the inner bag with a wire tie or blue rubber band. Avoid overfilling, as there is limited space in the autoclave. If the inner bag is damaged, seal the outer bag closed around the damaged inner bag.

        Using secondary containment and wearing appropriate PPE, bring the bagged waste to Room 142 or 143 in Building 100, or Room 418 in Building 400.

        Place all bags in the metal rectangular containers that are present in the rooms.

        Sharps containers in the lab are labeled as unregulated sharps, and should be placed in the metal containers after closing the sharps container lid. Do not overfill the sharps container. Once the sharps container is autoclaved, contact your Waste Management Generator Assistant for guidance on disposal of the sharps container.