Generally a 1% solution
of an iodophor decontaminant (Wescodyne or equivalent) is effective
against most viruses, fungi, vegetative bacteria, and most nonencysted
ameba.
A decontaminant detergent has the advantage of detergent activity,
which is important because extraneous organic substances frequently
interfere with the reaction between microorganisms and the active
agent of the decontaminant. Operator should wear gloves and lab
coat during this procedure.
Flood the top work surface tray, and, if a Class II Cabinet, the
drain pans and catch basins below the work surface, with a decontaminant
and allow to stand 10 to 15 minutes.
Remove excess decontaminant from the tray by wiping with a sponge
or cloth soaked in a decontaminant.
For Class II Cabinets, drain the tray into the Cabinet base, lift
out tray and removable exhaust grille work, and wipe off top and
bottom (underside) surfaces with a sponge or cloth soaked in a decontaminant.
Then replace in position and drain decontaminant from Cabinet base
into appropriate container and autoclave according to standard procedures.
Gloves, cloth, or sponge should be discarded in an autoclave pan
and autoclaved.
