|
PRACTICE
OR PROCEDURE
|
BL1
|
BL2*
|
BL3
|
|
Access
is limited or restricted at the discretion of the Principal
Investigator
|
Y
|
Y
|
Y
|
|
The
universal biohazard symbol must be posted on the access
door to the BL2 laboratory
|
N
|
Y
|
Y
|
|
Access
is through an airlock system.
|
N
|
N
|
Y
|
|
Access
to facility is limited or restricted during experiments.
|
N
|
Y
|
Y
|
|
Doors
to lab are kept closed when experimental animals are
present.
|
Y
|
Y
|
Y
|
|
Instructions
for safety precautions are posted on each door (as necessary)
by the PI
|
N
|
Y
|
Y
|
|
Requirements
for personnel protection are posted on each door (as
necessary)
|
Y
|
Y
|
Y
|
|
Personnel
wear goggles or face shields if possibility of splashes
and aerosols exist
|
Y
|
Y
|
Y
|
|
Laboratory
coats or gowns and gloves must be worn.
|
Y
|
Y
|
Y
|
|
Eating,
drinking, smoking, storing of food, inserting or removing
contact lenses and application of cosmetics is prohibited.
|
Y
|
Y
|
Y
|
|
Pipetting
by mouth is prohibited.
|
Y
|
Y
|
Y
|
|
Laboratory
coats or gowns and gloves must be worn.
|
Y
|
Y
|
Y
|
|
Personnel
must wash their hands after handling cultures and animals,
removing gloves and before leaving the facility
|
Y
|
Y
|
Y
|
|
Personnel
require specific training in the handling of pathogenic
materials
|
N
|
Y
|
Y
|
|
All
procedures should be performed to minimize aerosol generation
|
Y
|
Y
|
Y
|
|
Biosafety
cabinets are needed to prevent the release of aerosols
into lab
|
N
|
Y
|
Y
|
|
Work
surfaces must be decontaminated at least daily, after
each use for infrequent users and after any spill of
viable materials
|
Y
|
Y
|
Y
|
|
Sharps
must be placed in specially designed puncture and leak-proof
sharps containers and disposed of appropriately as medical
waste
|
Y
|
Y
|
Y
|
|
Laboratories
must be kept neat; good housekeeping procedures must
be in place and in regular use
|
Y
|
Y
|
Y
|
