Work Practices

WORK PRACTICES   

Centrifugation is performed in screw cap or pressure seal tubes/bottles which have been secondarily sealed with Parafilm (or equivalent).  If there has been any possibility of leakage, the inner walls of the centrifuge chamber and the rotor should be immediately decontaminated.  In the event that there are no safety centrifuge tubes or carriers available, the centrifuge can be placed in a biosafety cabinet.  It is important to place the centrifuge away from the front of the cabinet opening.

Plastic flasks and bottles are used whenever possible to avoid breakage.  Cotton plugged flasks are not considered open vessels as long as the plugs fit tightly, with no tendency to pop out.

Class II Cabinets are installed and certified in accordance with National Sanitation Foundation specifications.

Exhaust air volume (CFM) are to be maintained at design condition, regardless of changes in filter resistance.  In-line damper with manometer across filter and damper or equivalent monitoring system to be used.

Laboratories with supply air will have the supply air volume (CFM) controlled so as to assure inflow of air to laboratory from adjacent areas.

Ventilation systems are to be monitored on an annual basis by the EHS and records kept indicating airflow, filter resistance, and other pertinent data kept.

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