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Bloodborne Pathogens Retraining (EHS 0738)
EHS 0738 SITE MAP
BBP INTRODUCTION
BLOOD-BORNE PATHOGEN (BBP)
PREVENTION/
EXPOSURE CONTROL
INCIDENT RESPONSE
QUESTIONS
BIOSAFETY MANUAL
 
 

SAFETY EQUIPMENT (PRIMARY BARRIERS)

Properly maintained biological safety cabinets are used whenever procedures such as centrifugation, shaking, blending, sonication or opening containers in the presence of pressure differentials, inoculating animals intranasally, and harvesting infected tissues from animals are performed.

High concentration or large volumes of infectious agents are used.  Such materials may be centrifuged in the open laboratory if sealed heads or centrifuge safety cups are used and if they are opened only in a biological safety cabinet.

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Face protection, such goggles, mask, face shield or other splatter guards are used for anticipated splashes or sprays of infectious or other hazardous materials to the face, when the microorganisms are manipulated outside the BSC.

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Protective laboratory clothing designated for lab use is worn while in the laboratory. 

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This protective clothing is removed and left in the laboratory before leaving for non-laboratory areas.  All protective clothing is either disposed of in the laboratory or laundered by the institution; it should never be taken home by personnel.

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Gloves are worn when handling infected animals and when hands may contact potentially infectious or blood associated materials, contaminated surfaces, or equipment.  Wearing two pairs of gloves may be appropriate when handling HIV or materials known to contain HIV. Double gloves are of value when a spill or splatter occurs, the hand will be protected after the contaminated glove is removed.  Gloves are disposed of when contaminated, removed when work with infectious or rDNA materials is completed, and are not worn outside of the laboratory.  Disposable gloves are not washed or reused.

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