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SAFETY
EQUIPMENT (PRIMARY BARRIERS)
Properly maintained biological safety cabinets
are used whenever procedures such as centrifugation, shaking,
blending, sonication or opening containers in the presence
of pressure differentials, inoculating animals intranasally,
and harvesting infected tissues from animals are performed.
High concentration or large volumes of infectious
agents are used. Such materials may be centrifuged in
the open laboratory if sealed heads or centrifuge safety cups
are used and if they are opened only in a biological safety
cabinet.
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Face
protection, such goggles, mask, face shield or other splatter
guards are used for anticipated splashes or sprays of infectious
or other hazardous materials to the face, when the microorganisms
are manipulated outside the BSC.
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Protective
laboratory clothing designated for lab use is worn while in
the laboratory.
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This
protective clothing is removed and left in the laboratory
before leaving for non-laboratory areas. All protective
clothing is either disposed of in the laboratory or laundered
by the institution; it should never be taken home by personnel.
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Gloves
are worn when handling infected animals and when hands may contact
potentially infectious or blood associated materials, contaminated
surfaces, or equipment. Wearing two pairs of gloves may be
appropriate when handling HIV or materials known to contain HIV.
Double gloves are of value when a spill or splatter occurs, the
hand will be protected after the contaminated glove is removed.
Gloves are disposed of when contaminated, removed when work with
infectious or rDNA materials is completed, and are not worn outside
of the laboratory. Disposable gloves are not washed or reused.
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