Researchers at Berkeley Lab have developed a screening platform for quantitative profiling of colony organizations in three-dimensional (3D) cell culture models. The 3D cell culture models, which are also imaged in 3D, are functional assays that mimic the in vivo characteristics of tissue architecture more faithfuly than 2D cultures.
Evaluation and testing of this technology have been tightly coupled with the experimental design that consists of four cell lines with a significant genetic diversity and distinct phenotypical signatures. Comparison of the Berkeley Lab technology with alternative methods demonstrates superior performance, in terms of error rates. Furthermore, multiparametric representation for the colony organization has identified indices that capture aberrant and normal morphogenesis in these four cell lines.
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REFERENCE NUMBER: 2013-177