Ernest Orlando Lawrence Berkeley National Laboratory
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a, Metaphase chromosomes from
uninfected cell stained with anti-TIN2 (endogenous TIN2) antibody.
b, Metaphase chromosomes from Myc–TIN2-expressing cell
stained with anti-Myc (retroviral TIN2) antibody. c, Interphase
nucleus of an HA–TRF-1/Myc–TIN2-expressing cell stained
with anti-Myc (retroviral TIN2) antibody. d, Interphase nucleus
of the same HA–TRF-1/Myc–TIN2-expressing cell stained
with anti-HA (retroviral TRF1) antibody. e, Co-localization
of HA–TRF1 and Myc–TIN2 in nucleus shown in (c) and
(d) (merged image). f, DAPI staining of nucleus shown in (c–e).
g, Interphase nucleus of another HA–TRF-1/Myc–TIN2-expressing
cell stained with anti-Myc (retroviral TIN2) antibody. h, Interphase
nucleus of the same HA–TRF-1/Myc–TIN2-expressing cells
stained with anti-HA (retroviral TRF1) antibody. i, Co-localization
of HA–TRF1/Myc–TIN2 in nucleus shown in (g) and (h)
(merged image). j, DAPI staining of nucleus shown in (g–i).
k, Metaphase chromosomes from HA–TRF1/Myc–TIN2-13–expressing
cell stained with anti-HA antibody (telomeric localization of
TRF1 in the presence of TIN2-13). l, Metaphase chromosomes from
HA–TRF1/Myc–TIN2-13–expressing cells stained
with anti-Myc antibody (telomeric localization of TIN2-13).
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APPLICATIONS OF TECHNOLOGY:
- Molecular biology research
- Medical research
ABSTRACT: Telomeres are believed to be critical regulators
of the genome, and telomere stability important for preventing cancer
and ageing. Regulation of telomere length is believed to be controlled
by multiple telomere-associated proteins. Using a previously cloned
human telomere binding protein, Berkeley Lab researchers Sahn-ho
Kim and Judith Campisi cloned two novel human telomere-associated
proteins, TANK-2 and TIN-2. The full length sequence of one, and
a partial sequence of the other is known. TANK-2 and TIN-2 may be
important telomere regulators, and therefore of interest in the
health care arena for the diagnosis or treatment of cancer or age-related
diseases.
STATUS: U.S. Patent #6,409,648. Available for licensing
PUBLICATIONS:
"TIN2, a new regulator
of telomere length in human cells," Sahn-ho Kim, Patrick Kaminker
& Judith Campisi, Nature volume 23 no. 4 pp 405 - 412
(1999)
REFERENCE NUMBER: IB-1317
