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Bissell Laboratory Protocols

Cell Culture, Immunohistochemistry/Immunofluorescence, Molecular Biology/Biochemistry, Mouse Mammary Gland Biology

Cell Culture Protocols

HMT-3522 Cell Media Composition

HMT-3522 Cell Density Information

HMT-3522 Passaging Cells

HMT-3522 Cell Feeding

EHS "On Top" Culture

EHS "3D" Culture

Methyl Cellulose

Anchorage Independence Assay using Methyl Cellulose

Invasion Assay

Soft Agar Assay for Colony Formation

Protein Lysis of "3D" Culture using PBS

Isolating Xenograft Tumor Cells for Tissue Culture or Transplantation

Immunohistochemistry/Immunofluorescence

BrdU Staining

Propidium Iodide Staining for DNA Ploidy

Occludin Staining

In Situ Hybridization on mammary Sections

Molecular and Biochemical Assays

FACS Analysis

RNA extraction

Chromatin Immunoprecipitation Assay

Mouse Mammary Gland Biology

Mouse mammary gland wholemounts

Primary Mammary Epithelial Cell Isolation (From the Mouse Mammary Gland)

 

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