2-HMT-3522 density

 

 

1-      volume

 

4 wells chamber slides = 1.7 cm2

4 wells = 2 cm2

35 mm = 9.62 cm2

T25 = 25 cm2

60 mm = 26.26 cm2 = 3 X 35mm

100 mm = 78.54 cm2 = 8 X 35mm

T75 = 75 cm2 = 7.8 X 35 mm or 3X T25

 

 

Total volume of medium (half with matrigel if OT culture)

Volume of matrigel for On top + %drip culture

Volume of matrigel for 3d culture

4 wells Chamber slides

0.3-0.4 ml

60 ml / 2 to 10% FV (20ml)

 

4 wells

0.5ml

 

0.3 ml

35 mm

1 - 2 ml

0.5 ml / 2 to 10% FV

1.2 ml

60 mm

3 5 ml

1.5 ml / 2 to 10% FV

3.6 ml

T25

4 - 5 ml

-

-

T75

10 ml

-

-

 

2-      density

 

S1 cells: 2X104 cells/cm2

T4-2 cells: 1X104 cells/cm2

 

a-                              S1 cells

 

 

Plastic (2d)

On top (OT)

Inside (3d)

4 Chamber slides

35000 cells per well

40000 cells per well

40000 cells per well

50000 cells per well

 

35 mm

9.62 cm2

0.2 M to 0.25 M

0.8 M

0.3 M

 

0.8M/ ml matrigel

60 mm or T25

26.26 cm2

0.5 to 0.65 M

 

2.4 M

0.9 M

 

100 mm or T 75

~ 75 cm2

1.5M to 1.75

 

 

 

 

 

 

b-                             T4-2 cells

 

 

Plastic (2d)

On top (OT)

Inside (3d)

4 Chamber slides

17000 cells per well

 

0.04 M

 

35 mm

9.62 cm2

0.08 M

0.2 M

 

0.6M/ ml matrigel

60 mm or T25

26.26 cm2

0.25 M

 

0.6 M

 

100 mm or T 75

~ 75 cm2

0.75 M

1.6 M